Make sure the setup for Eu Cryptate is used. Plate 25 μL of cells in. This protocol, HTS designed, allows miniaturization while. It features a modular design that allows users to add or change detection cartridges according to their needs. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Your lab might even have one already. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. To perform the assay, use white plate only. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. 0. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . + 4 µL HTRF pre-mixed antibodies. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. There are multiple factors to consider including: detection type, additional. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It can also be further streamlined to a one-. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. FOR HTRF CERTIFIED READER For. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. FOR HTRF CERTIFIED. View Brochure. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Excitation lasers significantly improve performance and lower. Figure 1. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. FOR HTRF CERTIFIED READER. Incubate 24 hours at RT. Its. HTRF is compatible with a large range of available microplate readers. Read & analyse. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 5. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. In this case the Z´(pronounced Z prime) value comes to mind. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. Microplate readers are only certified if they. Its. FOR HTRF CERTIFIED READER. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. At Cisbio we provide microplates that help you discover better. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information. It enables you to gain insights you couldn’t achieve with detection measurements alone. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. Phospho-Rb (Ser780) two-plate assay protocol. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. Revvity. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. FOR HTRF CERTIFIED READER. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). For more information about HTRF. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. DLReady™ certified luminometer plate readers. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. For more information about HTRF. Envision Nexus. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. The lysis buffer 4X is ready-to-use. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Incubate 2h at RT Transfer 16 µL supernatant. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. After an overnight incubation, the plate is washed 3 times. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Filters or monochromators for emission wavelength. As shown in Fig. 1 – 1. 9. • HTRF®-Certified Reader. best fluorescence polarization plate reader on the market. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 EXAMPLE OF MAP PLATE . These plates can be read on all HTRF certified readers, thus making their implementation in. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. View Brochure. It can also be further streamlined to a one-. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. These plates can be read on all HTRF certified readers, thus making their implementation in. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. FOR HTRF CERTIFIED READER. Read & analyse. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. FOR HTRF CERTIFIED READER . Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. As shown in Fig. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Highly compatible plates for HTRF. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. FOR HTRF CERTIFIED READER For more. The optical systems use two scanningHighly compatible plates for HTRF. FOR HTRF CERTIFIED READER. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. -CERTIFIED READER IS NEEDED. protocol with suspension cells or with a. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. This protocol gives the cells viability and confluence to be monitored. HTRF-specific. Each well requires 10 μL of 4X lysis buffer /well. At Cisbio we provide microplates that help you discover better. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The dual measurement. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. FOR HTRF CERTIFIED READER. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Due to the chemical and photophysical. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Highly compatible plates for HTRF. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. Add. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. The optional HTRF-certified filter-based module may be. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . • HTRF®-Certified Reader. Some vendors hold the entire market share for not only a. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Remove the plate seal and read on an HTRF compatible reader. To perform the assay, use white plate only. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The donor and acceptor can be used to label a variety of biomolecules, for applications. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. Page 30 4. Plate cells Stimulate cells 3. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. For more information about HTRF. Its. FOR HTRF CERTIFIED READER. S. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. . It can also be further streamlined to a one-. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Results for HTRF Reader Control Kit with black and white microplates. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Dual monochromators for assay flexibility With. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. This protocol enables the cells' viability and confluence. PerkinElmer. e. The mean and standard deviation. Remove the plate seal and read on an HTRF compatible reader. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. The controls are equivalent to the upper and lower limit of. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Remove the plate sealer and read on an HTRF® compatible reader . Up to 8 decades dynamic range in one measurement with EDR technology. FOR HTRF CERTIFIED READER. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. For fluorescent assays, black plates absorb light and reduce background and crosstalk. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Incubate overnight at RT. FOR HTRF CERTIFIED READER. Experimental details• HTRF®-Certified Reader **. Make sure the setup for Eu3+ Cryptate is used. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Assay setup for a 384-well low-volume plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. First the plate is coated with a capture antibody. Discover our high performance reader. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more. overnight at 4 C [Prostaglandin E2] pg/ml. For more information about HTRF ®Seal the plate and incubate for Overnight. FOR HTRF CERTIFIED READER For. and Canaa -8-963-4567. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Eu 3+ Cryptate is used. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. + 4 µL HTRF ® pre-mixed antibodies . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 5 µL Prostaglandin E2 . For more information. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Handle complex biological matrices easily. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. Incubate overnight at RT. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Eu cryptate emission level). Remove the plate sealer and read on an HTRF® compatible reader . Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 1. The assay is then ready to be read on an HTRF compatible or certified microplate reader. Partnerships for delivering turn-key solutions. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. SV detection plate. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. Figure 1: Principle of HTRF Cortisol competitive assay. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Alpha Technology: The VICTOR Nivo system. This protocol, HTS designed, allows miniaturization while. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate is set up for reading and images are acquired according to specified parameters. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. PerkinElmer. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. After defining the objectives and their locations in Gen5: 1. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Conclusion. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. Neocarzinostatin dose-response on Jurkat cells. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Make sure the setup for Tb Cryptate is used. For more. The Negative control is used to check the non-specific signal. , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). FOR HTRF CERTIFIED READER. Each well generally requires 50µL of lysis buffer 1X. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. $1,024. Read & analyse. Push the screw down and turn it clockwise to lock the plate. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information about HTRF ® compatible readers and for set-up recommendations, please. PerkinElmer EnVision Multimode Plate Reader. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. 96-well culture plateFOR HTRF CERTIFIED READER. Plate 25 μL of cells in. For a list of HTRF-compatible readers and set -up recommendations, please visit. Remove the plate seal and read on an HTRF compatible reader. FOR HTRF CERTIFIED READER. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Download Setup. Read & analyse. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. If not, our scientific team will help you choose the instrument that best suits your needs and budget. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. FOR HTRF CERTIFIED. Incubate overnight at RT. PURCHASE SEPARATELY . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Incubate 4h at RT. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. Your lab might even have one already. For more information. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER For more information about HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit . Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. + 4 µL HTRF pre-mixed antibodies. Make sure to use the set-up for Eu Cryptate. Read & analyse. FOR HTRF CERTIFIED READER For more information about HTRF. Remove the plate sealer and read on an HTRF® compatible reader . FOR HTRF CERTIFIED READER For more information about HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. Catalog No. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Read other about this measurement approach here. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. FOR HTRF CERTIFIED READER For. The assay was prepared in both white and black plates and incubated >18 hours. Simply place the module into one of the five module bays and read the assay. 14 seconds for a 384-well plate. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibo dies 2. It also integrates with the powerful SoftMax Pro Software for. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. HTRF certified . Microplate reader assays requiring infrared (IR) or. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. FOR HTRF CERTIFIED READER. No washing steps are required. Incubate overnight at RT. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plus . It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute.